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Cryo-EM structures of human P4-ATPase flippase

ABSTRACT:

SUBMITTER: Tomohiro Nishizawa

PROVIDER: EMPIAR-10303 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structures capture the transport cycle of the P4-ATPase flippase.

Hiraizumi Masahiro M Yamashita Keitaro K Nishizawa Tomohiro T Nureki Osamu O

Science (New York, N.Y.) 20190815 6458

In eukaryotic membranes, type IV P-type adenosine triphosphatases (P4-ATPases) mediate the translocation of phospholipids from the outer to the inner leaflet and maintain lipid asymmetry, which is critical for membrane trafficking and signaling pathways. Here, we report the cryo-electron microscopy structures of six distinct intermediates of the human ATP8A1-CDC50a heterocomplex at resolutions of 2.6 to 3.3 angstroms, elucidating the lipid translocation cycle of this P4-ATPase. ATP-dependent pho ...[more]

PMID: 31416931

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