FIB-SEM of a HeLa cell
Ontology highlight
ABSTRACT:
SUBMITTER: Yannick Schwab
PROVIDER: EMPIAR-10311 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
AMST: Alignment to Median Smoothed Template for Focused Ion Beam Scanning Electron Microscopy Image Stacks.
Hennies Julian J Lleti José Miguel Serra JMS Schieber Nicole L NL Templin Rachel M RM Steyer Anna M AM Schwab Yannick Y
Scientific reports 20200206 1
Alignment of stacks of serial images generated by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) is generally performed using translations only, either through slice-by-slice alignments with SIFT or alignment by template matching. However, limitations of these methods are two-fold: the introduction of a bias along the dataset in the z-direction which seriously alters the morphology of observed organelles and a missing compensation for pixel size variations inherent to the image acquisit ...[more]
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