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Apo-state Streptavidin

ABSTRACT:

SUBMITTER: Nieng Yan

PROVIDER: EMPIAR-10335 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy.

Han Yimo Y Fan Xiao X Wang Haozhe H Zhao Fang F Tully Christopher G CG Kong Jing J Yao Nan N Yan Nieng N

Proceedings of the National Academy of Sciences of the United States of America 20191226 2

Cryogenic electron microscopy (cryo-EM) has become one of the most powerful techniques to reveal the atomic structures and working mechanisms of biological macromolecules. New designs of the cryo-EM grids-aimed at preserving thin, uniform vitrified ice and improving protein adsorption-have been considered a promising approach to achieving higher resolution with the minimal amount of materials and data. Here, we describe a method for preparing graphene cryo-EM grids with up to 99% monolayer graph ...[more]

PMID: 31879346

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