Cardiac thin filament in low calcium state
Ontology highlight
ABSTRACT:
SUBMITTER: Toshiyuki Oda
PROVIDER: EMPIAR-10348 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.
Oda Toshiyuki T Yanagisawa Haruaki H Wakabayashi Takeyuki T
Journal of structural biology 20200116 3
Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We deter ...[more]
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