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Single Particle Cryo-EM Reconstructions of NCP-CHD4 complexes

ABSTRACT:

SUBMITTER: Patrick Cramer

PROVIDER: EMPIAR-10411 | biostudies-other |

REPOSITORIES: biostudies-other

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Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations.

Farnung Lucas L Ochmann Moritz M Cramer Patrick P

eLife 20200616

Chromatin remodeling plays important roles in gene regulation during development, differentiation and in disease. The chromatin remodeling enzyme CHD4 is a component of the NuRD and ChAHP complexes that are involved in gene repression. Here, we report the cryo-electron microscopy (cryo-EM) structure of <i>Homo sapiens</i> CHD4 engaged with a nucleosome core particle in the presence of the non-hydrolysable ATP analogue AMP-PNP at an overall resolution of 3.1 Å. The ATPase motor of CHD4 binds and ...[more]

PMID: 32543371

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