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Single-Particle CryoEM of Human Apoferritin Light Chain Vitrified Using Back-it-up

ABSTRACT:

SUBMITTER: Yong Zi Tan

PROVIDER: EMPIAR-10421 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Through-grid wicking enables high-speed cryoEM specimen preparation.

Tan Yong Zi YZ Rubinstein John L JL

Acta crystallographica. Section D, Structural biology 20201013 Pt 11

Blotting times for conventional cryoEM specimen preparation complicate time-resolved studies and lead to some specimens adopting preferred orientations or denaturing at the air-water interface. Here, it is shown that solution sprayed onto one side of a holey cryoEM grid can be wicked through the grid by a glass-fiber filter held against the opposite side, often called the `back', of the grid, producing a film suitable for vitrification. This process can be completed in tens of milliseconds. Ultr ...[more]

PMID: 33135680

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