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170314, Five-day-old Col-0 Arabidopsis thaliana root, phloem pole unloading zone (339-381 um from the root tip)

ABSTRACT:

SUBMITTER: Ilya Belevich

PROVIDER: EMPIAR-10442 | biostudies-other |

REPOSITORIES: biostudies-other

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Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading.

Yan Dawei D Yadav Shri Ram SR Paterlini Andrea A Nicolas William J WJ Petit Jules D JD Brocard Lysiane L Belevich Ilya I Grison Magali S MS Vaten Anne A Karami Leila L El-Showk Sedeer S Lee Jung-Youn JY Lee Jung-Youn JY Murawska Gosia M GM Mortimer Jenny J Knoblauch Michael M Jokitalo Eija E Markham Jennifer E JE Bayer Emmanuelle M EM Helariutta Ykä Y

Nature plants 20190610 6

During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanc ...[more]

PMID: 31182845

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Phloem unloading via the apoplastic pathway is essential for shoot distribution of root-synthesized cytokinins.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading.

Project description:During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (PLM), in the absence of which plasmodesmata-mediated symplastic transport through the phloem pole pericycle-endodermis interface is specifically enhanced. Increased unloading is attributable to a defect in the formation of the endoplasmic reticulum-plasma membrane tethers during plasmodesmal morphogenesis, resulting in the majority of pores lacking a visible cytoplasmic sleeve. PLM encodes a putative enzyme required for the biosynthesis of sphingolipids with very-long-chain fatty acid. Taken together, our results indicate that post-sieve element unloading involves sphingolipid metabolism, which affects plasmodesmal ultrastructure. They also raise the question of how and why plasmodesmata with no cytoplasmic sleeve facilitate molecular trafficking.

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