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EER movies of human light chain apoferritin

ABSTRACT:

SUBMITTER: John L Rubinstein

PROVIDER: EMPIAR-10474 | biostudies-other |

REPOSITORIES: biostudies-other

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Electron-event representation data enable efficient cryoEM file storage with full preservation of spatial and temporal resolution.

Guo Hui H Franken Erik E Deng Yuchen Y Benlekbir Samir S Singla Lezcano Garbi G Janssen Bart B Yu Lingbo L Ripstein Zev A ZA Tan Yong Zi YZ Rubinstein John L JL

IUCrJ 20200807 Pt 5

Direct detector device (DDD) cameras have revolutionized electron cryomicroscopy (cryoEM) with their high detective quantum efficiency (DQE) and output of movie data. A high ratio of camera frame rate (frames per second) to camera exposure rate (electrons per pixel per second) allows electron counting, which further improves the DQE and enables the recording of super-resolution information. Movie output also allows the correction of specimen movement and compensation for radiation damage. Howeve ...[more]

PMID: 32939278

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