Cryo-EM structure of the SpCas9 delta4CE Ternary Complex
Ontology highlight
ABSTRACT:
SUBMITTER: Thomas Gerard Laughlin
PROVIDER: EMPIAR-10508 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules.
Shams Arik A Higgins Sean A SA Fellmann Christof C Laughlin Thomas G TG Oakes Benjamin L BL Lew Rachel R Kim Shin S Lukarska Maria M Arnold Madeline M Staahl Brett T BT Doudna Jennifer A JA Savage David F DF
Nature communications 20210927 1
Proteins evolve through the modular rearrangement of elements known as domains. Extant, multidomain proteins are hypothesized to be the result of domain accretion, but there has been limited experimental validation of this idea. Here, we introduce a technique for genetic minimization by iterative size-exclusion and recombination (MISER) for comprehensively making all possible deletions of a protein. Using MISER, we generate a deletion landscape for the CRISPR protein Cas9. We find that the catal ...[more]
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