Cryo electron microscopy of RagAB from P. gingivalis solubilised in DDM
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ABSTRACT:
SUBMITTER: Bert van den Berg
PROVIDER: EMPIAR-10543 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural and functional insights into oligopeptide acquisition by the RagAB transporter from Porphyromonas gingivalis.
Madej Mariusz M White Joshua B R JBR Nowakowska Zuzanna Z Rawson Shaun S Scavenius Carsten C Enghild Jan J JJ Bereta Grzegorz P GP Pothula Karunakar K Kleinekathoefer Ulrich U Baslé Arnaud A Ranson Neil A NA Potempa Jan J van den Berg Bert B
Nature microbiology 20200511 8
Porphyromonas gingivalis, an asaccharolytic member of the Bacteroidetes, is a keystone pathogen in human periodontitis that may also contribute to the development of other chronic inflammatory diseases. P. gingivalis utilizes protease-generated peptides derived from extracellular proteins for growth, but how these peptides enter the cell is not clear. Here, we identify RagAB as the outer-membrane importer for these peptides. X-ray crystal structures show that the transporter forms a dimeric RagA ...[more]
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