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The cryo-EM structure of the CENP-A nucleosome in complex with the phosphorylated CENP-C: CENP-A nucleosome in complex with phosphorylated CENP-C C-terminal domain (601-864) and CENP-N N-terminal domain (1-211)

ABSTRACT:

SUBMITTER: Fukagawa Tatsuo

PROVIDER: EMPIAR-10565 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C.

Ariyoshi Mariko M Makino Fumiaki F Watanabe Reito R Nakagawa Reiko R Kato Takayuki T Namba Keiichi K Arimura Yasuhiro Y Fujita Risa R Kurumizaka Hitoshi H Okumura Ei-Ichi EI Hara Masatoshi M Fukagawa Tatsuo T

The EMBO journal 20210119 5

The CENP-A nucleosome is a key structure for kinetochore assembly. Once the CENP-A nucleosome is established in the centromere, additional proteins recognize the CENP-A nucleosome to form a kinetochore. CENP-C and CENP-N are CENP-A binding proteins. We previously demonstrated that vertebrate CENP-C binding to the CENP-A nucleosome is regulated by CDK1-mediated CENP-C phosphorylation. However, it is still unknown how the phosphorylation of CENP-C regulates its binding to CENP-A. It is also not co ...[more]

PMID: 33463726

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