Cryo electron microscopy single particle of PIKfyve lipid kinase complex
Ontology highlight
ABSTRACT:
SUBMITTER: Joshua A Lees
PROVIDER: EMPIAR-10578 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Insights into Lysosomal PI(3,5)P<sub>2</sub> Homeostasis from a Structural-Biochemical Analysis of the PIKfyve Lipid Kinase Complex.
Lees Joshua A JA Li PeiQi P Kumar Nikit N Weisman Lois S LS Reinisch Karin M KM
Molecular cell 20201023 4
The phosphoinositide PI(3,5)P<sub>2</sub>, generated exclusively by the PIKfyve lipid kinase complex, is key for lysosomal biology. Here, we explore how PI(3,5)P<sub>2</sub> levels within cells are regulated. We find the PIKfyve complex comprises five copies of the scaffolding protein Vac14 and one copy each of the lipid kinase PIKfyve, generating PI(3,5)P<sub>2</sub> from PI3P and the lipid phosphatase Fig4, reversing the reaction. Fig4 is active as a lipid phosphatase in the ternary complex, w ...[more]
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