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Cryo Soft X-ray data for tetraspeck correlation


ABSTRACT:

SUBMITTER: Johannes Groen 

PROVIDER: EMPIAR-10615 | biostudies-other |

REPOSITORIES: biostudies-other

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Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.

Okolo Chidinma A CA   Kounatidis Ilias I   Groen Johannes J   Nahas Kamal L KL   Balint Stefan S   Fish Thomas M TM   Koronfel Mohamed A MA   Cortajarena Aitziber L AL   Dobbie Ian M IM   Pereiro Eva E   Harkiolaki Maria M  

Nature protocols 20210514 6


3D correlative microscopy methods have revolutionized biomedical research, allowing the acquisition of multidimensional information to gain an in-depth understanding of biological systems. With the advent of relevant cryo-preservation methods, correlative imaging of cryogenically preserved samples has led to nanometer resolution imaging (2-50 nm) under harsh imaging regimes such as electron and soft X-ray tomography. These methods have now been combined with conventional and super-resolution flu  ...[more]

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