Cryo-EM dataset of human pre-Bact spliceosome
Ontology highlight
ABSTRACT:
SUBMITTER: Cole Townsend
PROVIDER: EMPIAR-10616 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.
Townsend Cole C Leelaram Majety N MN Agafonov Dmitry E DE Dybkov Olexandr O Will Cindy L CL Bertram Karl K Urlaub Henning H Kastner Berthold B Stark Holger H Lührmann Reinhard R
Science (New York, N.Y.) 20201126 6523
Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-electron microscopy structures of two human, activated spliceosome precursors (that is, pre-B<sup>act</sup> complexes) at core resolutions of 3.9 and 4.2 angstroms. These structures elucidate the order of ...[more]
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