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Cryo-electron tomography of HIV-1 GagdeltaMASP1T8I assemblies


ABSTRACT:

SUBMITTER: Luiza Montenegro Mendonca 

PROVIDER: EMPIAR-10643 | biostudies-other |

REPOSITORIES: biostudies-other

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High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.

Ni Tao T   Frosio Thomas T   Mendonça Luiza L   Sheng Yuewen Y   Clare Daniel D   Himes Benjamin A BA   Zhang Peijun P  

Nature protocols 20220112 2


Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macromolecular complexes in situ and in cellular context at or below subnanometer resolution and has led to unprecedented insights into the inner working of molecular machines in their native environment, as well as their functional relevant conformations and spatial distribution within biological cells or tissues. Given the tremendous potential of cryo-electron tomography ST  ...[more]

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