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Affinity-purified LsaA in complex with 70S ribosomes from Enterococcus faecalis

ABSTRACT:

SUBMITTER: Caillan Thomas Crowe-McAuliffe

PROVIDER: EMPIAR-10682 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.

Crowe-McAuliffe Caillan C Murina Victoriia V Turnbull Kathryn Jane KJ Kasari Marje M Mohamad Merianne M Polte Christine C Takada Hiraku H Vaitkevicius Karolis K Johansson Jörgen J Ignatova Zoya Z Atkinson Gemma C GC O'Neill Alex J AJ Hauryliuk Vasili V Wilson Daniel N DN

Nature communications 20210611 1

Target protection proteins confer resistance to the host organism by directly binding to the antibiotic target. One class of such proteins are the antibiotic resistance (ARE) ATP-binding cassette (ABC) proteins of the F-subtype (ARE-ABCFs), which are widely distributed throughout Gram-positive bacteria and bind the ribosome to alleviate translational inhibition from antibiotics that target the large ribosomal subunit. Here, we present single-particle cryo-EM structures of ARE-ABCF-ribosome compl ...[more]

PMID: 34117249

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