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In situ cryo-electron tomogram of a pyrenoid inside a Chlamydomonas reinhardtii cell (tilt series)

ABSTRACT:

SUBMITTER: Ricardo D. Righetto

PROVIDER: EMPIAR-10694 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.

Freeman Rosenzweig Elizabeth S ES Xu Bin B Kuhn Cuellar Luis L Martinez-Sanchez Antonio A Schaffer Miroslava M Strauss Mike M Cartwright Heather N HN Ronceray Pierre P Plitzko Jürgen M JM Förster Friedrich F Wingreen Ned S NS Engel Benjamin D BD Mackinder Luke C M LCM Jonikas Martin C MC

Cell 20170901 1

Approximately 30%-40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell d ...[more]

PMID: 28938114

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