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Mixed capsids of MS2 virions


ABSTRACT:

SUBMITTER: Christopher Herbert Stanley Aylett 

PROVIDER: EMPIAR-10706 | biostudies-other |

REPOSITORIES: biostudies-other

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Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids.

de Martín Garrido Natàlia N   Crone Michael A MA   Ramlaul Kailash K   Simpson Paul A PA   Freemont Paul S PS   Aylett Christopher H S CHS  

Molecular microbiology 20191105 1


Bacteriophage MS2 is a positive-sense, single-stranded RNA virus encapsulated in an asymmetric T = 3 pseudo-icosahedral capsid. It infects Escherichia coli through the F-pilus, in which it binds through a maturation protein incorporated into its capsid. Cryogenic electron microscopy has previously shown that its genome is highly ordered within virions, and that it regulates the assembly process of the capsid. In this study, we have assembled recombinant MS2 capsids with non-genomic RNA containin  ...[more]

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