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Mono nucleosome fraction from interphase chromosome in Xenopus egg extract lot1


ABSTRACT:

SUBMITTER: Hironori Funabiki 

PROVIDER: EMPIAR-10742 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural features of nucleosomes in interphase and metaphase chromosomes.

Arimura Yasuhiro Y   Shih Rochelle M RM   Froom Ruby R   Funabiki Hironori H  

Molecular cell 20210902 21


Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo-EM structures of protein complexes from interphase or metaphase chromosomes. The reconstructed interphase and metaphase nucleosome structures are on average indistinguishable from canonical nucleosome structures, despite DNA sequence heterogeneity, cell-cycle-specific posttranslational modifications,  ...[more]

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