High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells
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ABSTRACT:
SUBMITTER: Andriko von Kügelgen
PROVIDER: EMPIAR-10790 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Herdman Matthew M von Kügelgen Andriko A Kureisaite-Ciziene Danguole D Duman Ramona R El Omari Kamel K Garman Elspeth F EF Kjaer Andreas A Kolokouris Dimitrios D Löwe Jan J Wagner Armin A Stansfeld Phillip J PJ Bharat Tanmay A M TAM
Structure (London, England : 1993) 20211119 2
Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. ...[more]
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