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A microtubule RELION-based pipeline for cryo-EM image processing


ABSTRACT:

SUBMITTER: Joseph Michael Atherton 

PROVIDER: EMPIAR-10795 | biostudies-other |

REPOSITORIES: biostudies-other

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A microtubule RELION-based pipeline for cryo-EM image processing.

Cook Alexander D AD   Manka Szymon W SW   Wang Su S   Moores Carolyn A CA   Atherton Joseph J  

Journal of structural biology 20191011 1


Microtubules are polar filaments built from αβ-tubulin heterodimers that exhibit a range of architectures in vitro and in vivo. Tubulin heterodimers are arranged helically in the microtubule wall but many physiologically relevant architectures exhibit a break in helical symmetry known as the seam. Noisy 2D cryo-electron microscopy projection images of pseudo-helical microtubules therefore depict distinct but highly similar views owing to the high structural similarity of α- and β-tubulin. The de  ...[more]

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