CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
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ABSTRACT:
SUBMITTER: Rebecca Conners
PROVIDER: EMPIAR-10807 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage.
Conners Rebecca R McLaren Mathew M Łapińska Urszula U Sanders Kelly K Stone M Rhia L MRL Blaskovich Mark A T MAT Pagliara Stefano S Daum Bertram B Rakonjac Jasna J Gold Vicki A M VAM
Nature communications 20211102 1
The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane ...[more]
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