Dataset Information
CryoEM single particle dataset for NanR dimer-DNA hetero-complex.
Ontology highlight
ABSTRACT:
SUBMITTER: Hari Venugopal
PROVIDER: EMPIAR-10836 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism.
Nature communications 20210331 1
Bacteria respond to environmental changes by inducing transcription of some genes and repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for pathogenic and commensal bacteria. The Escherichia coli GntR-type transcriptional repressor, NanR, regulates sialic acid metabolism, but the mechanism is unclear. Here, we demonstrate that three NanR dimers bind a (GGTATA)<sub>3</sub>-repeat operator cooperatively and with high affinity. Single-particle cryo-electron micr ...[more]