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Reconstructed cryo soft X-ray tomography dataset of a treated NIH-3T3 cell with corresponding correlated cryo-3D-SIM channels

ABSTRACT:

SUBMITTER: Johannes Groen

PROVIDER: EMPIAR-10839 | biostudies-other |

REPOSITORIES: biostudies-other

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Correlative 3D cryo X-ray imaging reveals intracellular location and effect of designed antifibrotic protein-nanomaterial hybrids.

Groen J J Palanca A A Aires A A Conesa J J JJ Maestro D D Rehbein S S Harkiolaki M M Villar A V AV Cortajarena A L AL Pereiro E E

Chemical science 20211019 45

Revealing the intracellular location of novel therapeutic agents is paramount for the understanding of their effect at the cell ultrastructure level. Here, we apply a novel correlative cryo 3D imaging approach to determine the intracellular fate of a designed protein-nanomaterial hybrid with antifibrotic properties that shows great promise in mitigating myocardial fibrosis. Cryo 3D structured illumination microscopy (cryo-3D-SIM) pinpoints the location and cryo soft X-ray tomography (cryo-SXT) r ...[more]

PMID: 34909150

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