Dataset Information
Single-particle cryoEM data of a ternary KRas(G13D)-SOS complex
Ontology highlight
ABSTRACT:
SUBMITTER: Minglei Zhao
PROVIDER: EMPIAR-10894 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants.
Proceedings of the National Academy of Sciences of the United States of America 20210301 12
Ras is regulated by a specific guanine nucleotide exchange factor Son of Sevenless (SOS), which facilitates the exchange of inactive, GDP-bound Ras with GTP. The catalytic activity of SOS is also allosterically modulated by an active Ras (Ras-GTP). However, it remains poorly understood how oncogenic Ras mutants interact with SOS and modulate its activity. Here, native ion mobility-mass spectrometry is employed to monitor the assembly of the catalytic domain of SOS (SOS<sup>cat</sup>) with KRas a ...[more]