Unknown

Dataset Information

0

CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the Fdx and upstream fork DNA

ABSTRACT:

SUBMITTER: Elizabeth A Campbell 

PROVIDER: EMPIAR-10901 | biostudies-other |

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Fidaxomicin jams <i>Mycobacterium tuberculosis</i> RNA polymerase motions needed for initiation via RbpA contacts.

Boyaci Hande H   Chen James J   Lilic Mirjana M   Palka Margaret M   Mooney Rachel Anne RA   Landick Robert R   Darst Seth A SA   Campbell Elizabeth A EA  

eLife 20180226

Fidaxomicin (Fdx) is an antimicrobial RNA polymerase (RNAP) inhibitor highly effective against <i>Mycobacterium tuberculosis</i> RNAP in vitro, but clinical use of Fdx is limited to treating <i>Clostridium difficile</i> intestinal infections due to poor absorption. To identify the structural determinants of Fdx binding to RNAP, we determined the 3.4 Å cryo-electron microscopy structure of a complete <i>M. tuberculosis</i> RNAP holoenzyme in complex with Fdx. We find that the actinobacteria gener  ...[more]

Similar Datasets

Unknown CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA
| EMPIAR-10896 | biostudies-other
Unknown CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA/Fdx
| EMPIAR-10887 | biostudies-other
Unknown CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to an inhibitor corallopyronin and us-fork DNA
| EMPIAR-10897 | biostudies-other
Unknown CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the de novo melted AP3 promoter DNA
| EMPIAR-10900 | biostudies-other
Unknown cryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme bound to the Mtb WhiB7 promoter
| EMPIAR-10864 | biostudies-other
Unknown CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to an inhibitor corallopyronin and de novo melted AP3 promoter DNA
| EMPIAR-10899 | biostudies-other
Unknown cryoEM of Mycobacterium tuberculosis WT RNAP holo enzyme with WhiB7 transcription factor bound to the Mtb WhiB7 promoter
| EMPIAR-10851 | biostudies-other
Unknown Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the σ A-containing RNA polymerase holoenzyme.
| S-EPMC3413145 | biostudies-literature
Unknown Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts.
| S-EPMC5837556 | biostudies-literature
Unknown Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD.
| S-EPMC5009747 | biostudies-literature