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CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the Fdx and upstream fork DNA

ABSTRACT:

SUBMITTER: Elizabeth A Campbell 

PROVIDER: EMPIAR-10901 | biostudies-other |

REPOSITORIES: biostudies-other

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Fidaxomicin jams <i>Mycobacterium tuberculosis</i> RNA polymerase motions needed for initiation via RbpA contacts.

Boyaci Hande H   Chen James J   Lilic Mirjana M   Palka Margaret M   Mooney Rachel Anne RA   Landick Robert R   Darst Seth A SA   Campbell Elizabeth A EA  

eLife 20180226

Fidaxomicin (Fdx) is an antimicrobial RNA polymerase (RNAP) inhibitor highly effective against <i>Mycobacterium tuberculosis</i> RNAP in vitro, but clinical use of Fdx is limited to treating <i>Clostridium difficile</i> intestinal infections due to poor absorption. To identify the structural determinants of Fdx binding to RNAP, we determined the 3.4 Å cryo-electron microscopy structure of a complete <i>M. tuberculosis</i> RNAP holoenzyme in complex with Fdx. We find that the actinobacteria gener  ...[more]

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