Apoferritin on EG-grid
Ontology highlight
ABSTRACT:
SUBMITTER: Junso Fujita
PROVIDER: EMPIAR-10953 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis.
Fujita Junso J Makino Fumiaki F Asahara Haruyasu H Moriguchi Maiko M Kumano Shota S Anzai Itsuki I Kishikawa Jun-Ichi JI Matsuura Yoshiharu Y Kato Takayuki T Namba Keiichi K Inoue Tsuyoshi T
Scientific reports 20230208 1
Functionalization of graphene is one of the most important fundamental technologies in a wide variety of fields including industry and biochemistry. We have successfully achieved a novel oxidative modification of graphene using photoactivated ClO<sub>2</sub><sup>·</sup> as a mild oxidant and confirmed the oxidized graphene grid is storable with its functionality for at least three months under N<sub>2</sub> atmosphere. Subsequent chemical functionalization enabled us to develop an epoxidized gra ...[more]
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Project description:Therapeutic outcome for the treatment of glioma was often limited due to the non-targeted nature of drugs and the physiological barriers, including the blood-brain barrier (BBB) and the blood-brain tumor barrier (BBTB). An ideal glioma-targeted delivery system must be sufficiently potent to cross the BBB and BBTB and then target glioma cells with adequate optimized physiochemical properties and biocompatibility. However, it is an enormous challenge to the researchers to engineer the above-mentioned features into a single nanocarrier particle. New frontiers in nanomedicine are advancing the research of new biomaterials. In this study, we demonstrate a strategy for glioma targeting by encapsulating vincristine sulfate (VCR) into a naturally available apoferritin nanocage-based drug delivery system with the modification of GKRK peptide ligand (GKRK-APO). Apoferritin (APO), an endogenous nanosize spherical protein, can specifically bind to brain endothelial cells and glioma cells via interacting with the transferrin receptor 1 (TfR1). GKRK is a peptide ligand of heparan sulfate proteoglycan (HSPG) over-expressed on angiogenesis and glioma, presenting excellent glioma-homing property. By combining the dual-targeting delivery effect of GKRK peptide and parent APO, GKRK-APO displayed higher glioma localization than that of parent APO. After loading with VCR, GKRK-APO showed the most favorable antiglioma effect in vitro and in vivo. These results demonstrated that GKRK-APO is an important potential drug delivery system for glioma-targeted therapy.
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| S-EPMC6119502 | biostudies-literature
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