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Dataset Information

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1.6Å Apoferritin from 200kV Glacios with Selectris Falcon 4


ABSTRACT:

SUBMITTER: Abhay Kotecha 

PROVIDER: EMPIAR-10973 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope.

Koh Adrian A   Khavnekar Sagar S   Yang Wen W   Karia Dimple D   Cats Dennis D   van der Ploeg Rob R   Grollios Fanis F   Raschdorf Oliver O   Kotecha Abhay A   Němeček Daniel D  

Journal of visualized experiments : JoVE 20220316 181


Cryo-electron microscopy (cryo-EM) has been established as a routine method for protein structure determination during the past decade, taking an ever-increasing share of published structural data. Recent advances in TEM technology and automation have boosted both the speed of data collection and quality of acquired images while simultaneously decreasing the required level of expertise for obtaining cryo-EM maps at sub-3 Å resolutions. While most of such high-resolution structures have been obta  ...[more]

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