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Cryo-EM images of equine apo-ferritin recorded as demonstration of MeasureIce software

ABSTRACT:

SUBMITTER: Hamish Galloway Brown

PROVIDER: EMPIAR-11013 | biostudies-other |

REPOSITORIES: biostudies-other

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MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy.

Brown Hamish G HG Hanssen Eric E

Communications biology 20220815 1

Ice thickness is arguably one of the most important factors limiting the resolution of protein structures determined by cryo-electron microscopy (cryo-EM). The amorphous atomic structure of the ice that stabilizes and protects biological samples in cryo-EM grids also imprints some additional noise in cryo-EM images. Ice that is too thick jeopardizes the success of particle picking and reconstruction of the biomolecule in the worst case and, at best, deteriorates eventual map resolution. Minimizi ...[more]

PMID: 35965271

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