Cryo-EM images of equine apo-ferritin recorded as demonstration of MeasureIce software
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ABSTRACT:
SUBMITTER: Hamish Galloway Brown
PROVIDER: EMPIAR-11013 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy.
Brown Hamish G HG Hanssen Eric E
Communications biology 20220815 1
Ice thickness is arguably one of the most important factors limiting the resolution of protein structures determined by cryo-electron microscopy (cryo-EM). The amorphous atomic structure of the ice that stabilizes and protects biological samples in cryo-EM grids also imprints some additional noise in cryo-EM images. Ice that is too thick jeopardizes the success of particle picking and reconstruction of the biomolecule in the worst case and, at best, deteriorates eventual map resolution. Minimizi ...[more]
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