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Cryo electron microscopy of in vitro recombinant SAA1.1 amyloid fibrils

ABSTRACT:

SUBMITTER: Matthias Schmidt

PROVIDER: EMPIAR-11027 | biostudies-other |

REPOSITORIES: biostudies-other

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AA amyloid fibrils from diseased tissue are structurally different from in vitro formed SAA fibrils.

Bansal Akanksha A Schmidt Matthias M Rennegarbe Matthies M Haupt Christian C Liberta Falk F Stecher Sabrina S Puscalau-Girtu Ioana I Biedermann Alexander A Fändrich Marcus M

Nature communications 20210212 1

Systemic AA amyloidosis is a world-wide occurring protein misfolding disease of humans and animals. It arises from the formation of amyloid fibrils from serum amyloid A (SAA) protein. Using cryo electron microscopy we here show that amyloid fibrils which were purified from AA amyloidotic mice are structurally different from fibrils formed from recombinant SAA protein in vitro. Ex vivo amyloid fibrils consist of fibril proteins that contain more residues within their ordered parts and possess a h ...[more]

PMID: 33579941

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