Dataset Information
Cryo iDPC-STEM single particle analysis of keyhole limpet hemocyanin
Ontology highlight
ABSTRACT:
SUBMITTER: Carsten Sachse
PROVIDER: EMPIAR-11034 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.
Nature methods 20220905 9
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC-STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV ...[more]