Cryo-EM data and 2DTM results of entire sections of differentiated ER-HoxB8 cells
Ontology highlight
ABSTRACT:
SUBMITTER: Johannes Elferich
PROVIDER: EMPIAR-11063 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sections.
Elferich Johannes J Schiroli Giulia G Scadden David T DT Grigorieff Nikolaus N
eLife 20221116
A major goal of biological imaging is localization of biomolecules inside a cell. Fluorescence microscopy can localize biomolecules inside whole cells and tissues, but its ability to count biomolecules and accuracy of the spatial coordinates is limited by the wavelength of visible light. Cryo-electron microscopy (cryo-EM) provides highly accurate position and orientation information of biomolecules but is often confined to small fields of view inside a cell, limiting biological context. In this ...[more]
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