In situ cryo-electron tomography of the C. reinhardtii ciliary transition zone
Ontology highlight
ABSTRACT:
SUBMITTER: Ricardo Diogo Righetto
PROVIDER: EMPIAR-11078 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains.
van den Hoek Hugo H Klena Nikolai N Jordan Mareike A MA Alvarez Viar Gonzalo G Righetto Ricardo D RD Schaffer Miroslava M Erdmann Philipp S PS Wan William W Geimer Stefan S Plitzko Jürgen M JM Baumeister Wolfgang W Pigino Gaia G Hamel Virginie V Guichard Paul P Engel Benjamin D BD
Science (New York, N.Y.) 20220728 6605
The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal ...[more]
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