Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex
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ABSTRACT:
SUBMITTER: Tsukasa Kusakizako
PROVIDER: EMPIAR-11110 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structure and engineering of the minimal type VI CRISPR-Cas13bt3.
Nakagawa Ryoya R Kannan Soumya S Altae-Tran Han H Takeda Satoru N SN Tomita Atsuhiro A Hirano Hisato H Kusakizako Tsukasa T Nishizawa Tomohiro T Yamashita Keitaro K Zhang Feng F Nishimasu Hiroshi H Nureki Osamu O
Molecular cell 20220825 17
Type VI CRISPR-Cas13 effector enzymes catalyze RNA-guided RNA cleavage and have been harnessed for various technologies, such as RNA detection, targeting, and editing. Recent studies identified Cas13bt3 (also known as Cas13X.1) as a miniature Cas13 enzyme, which can be used for knockdown and editing of target transcripts in mammalian cells. However, the action mechanism of the compact Cas13bt3 remains unknown. Here, we report the structures of the Cas13bt3-guide RNA complex and the Cas13bt3-guid ...[more]
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