Cryo electron tomography of H. neapolitanus alpha-carboxysomes
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ABSTRACT:
SUBMITTER: Grant J Jensen
PROVIDER: EMPIAR-11125 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Rubisco forms a lattice inside alpha-carboxysomes.
Metskas Lauren Ann LA Ortega Davi D Oltrogge Luke M LM Blikstad Cecilia C Lovejoy Derik R DR Laughlin Thomas G TG Savage David F DF Jensen Grant J GJ
Nature communications 20220818 1
Despite the importance of microcompartments in prokaryotic biology and bioengineering, structural heterogeneity has prevented a complete understanding of their architecture, ultrastructure, and spatial organization. Here, we employ cryo-electron tomography to image α-carboxysomes, a pseudo-icosahedral microcompartment responsible for carbon fixation. We have solved a high-resolution subtomogram average of the Rubisco cargo inside the carboxysome, and determined the arrangement of the enzyme. We ...[more]
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