In situ cryo-electron tomography of autophagic structures in S. cerevisiae
Ontology highlight
ABSTRACT:
SUBMITTER: Cristina Capitanio
PROVIDER: EMPIAR-11166 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
In situ structural analysis reveals membrane shape transitions during autophagosome formation.
Bieber Anna A Capitanio Cristina C Erdmann Philipp S PS Fiedler Fabian F Beck Florian F Lee Chia-Wei CW Li Delong D Hummer Gerhard G Schulman Brenda A BA Baumeister Wolfgang W Wilfling Florian F
Proceedings of the National Academy of Sciences of the United States of America 20220919 39
Autophagosomes are unique organelles that form de novo as double-membrane vesicles engulfing cytosolic material for destruction. Their biogenesis involves membrane transformations of distinctly shaped intermediates whose ultrastructure is poorly understood. Here, we combine cell biology, correlative cryo-electron tomography (cryo-ET), and extensive data analysis to reveal the step-by-step structural progression of autophagosome biogenesis at high resolution directly within yeast cells. The analy ...[more]
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