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Unaligned and aligned cryo-EM micrographs of 82-kDa malate synthase G

ABSTRACT:

SUBMITTER: Kuen-Phon Wu

PROVIDER: EMPIAR-11167 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM reveals the structure and dynamics of a 723-residue malate synthase G.

Ho Meng-Ru MR Wu Yi-Ming YM Lu Yen-Chen YC Ko Tzu-Ping TP Wu Kuen-Phon KP

Journal of structural biology 20230328 2

Determination of sub-100 kDa (kDa) structures by cryo-electron microscopy (EM) is a longstanding but not straightforward goal. Here, we present a 2.9-Å cryo-EM structure of a 723-amino acid apo-form malate synthase G (MSG) from Escherichia coli. The cryo-EM structure of the 82-kDa MSG exhibits the same global folding as structures resolved by crystallography and nuclear magnetic resonance (NMR) spectroscopy, and the crystal and cryo-EM structures are indistinguishable. Analyses of MSG dynamics r ...[more]

PMID: 36997036

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