Dataset Information
Single-particle cryo-EM dataset of ALC1 bound to an asymmetric, site-specifically PARylated nucleosome
Ontology highlight
ABSTRACT:
SUBMITTER: Guillaume Gaullier
PROVIDER: EMPIAR-11211 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Asymmetric nucleosome PARylation at DNA breaks mediates directional nucleosome sliding by ALC1.
Nature communications 20240202 1
The chromatin remodeler ALC1 is activated by DNA damage-induced poly(ADP-ribose) deposited by PARP1/PARP2 and their co-factor HPF1. ALC1 has emerged as a cancer drug target, but how it is recruited to ADP-ribosylated nucleosomes to affect their positioning near DNA breaks is unknown. Here we find that PARP1/HPF1 preferentially initiates ADP-ribosylation on the histone H2B tail closest to the DNA break. To dissect the consequences of such asymmetry, we generate nucleosomes with a defined ADP-ribo ...[more]