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Mouse heavy chain apoferritin in vitreous ice after laser-melting and revitrification


ABSTRACT:

SUBMITTER: Ulrich Lorenz 

PROVIDER: EMPIAR-11213 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach.

Bongiovanni Gabriele G   Harder Oliver F OF   Drabbels Marcel M   Lorenz Ulrich J UJ  

Frontiers in molecular biosciences 20221110


We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles to occur. Once the laser beam is switched off, the sample revitrifies within just a few microseconds, trapping the particles in their transient configurations, which can subsequently be imaged to obtain a snap shot of the dynamics at this point in time. While we ha  ...[more]

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