RedBeta177 oligomeric helical assembly bound to two complementary 27mer ssDNA oligonucleotides
Ontology highlight
ABSTRACT:
SUBMITTER: Gökhan Tolun
PROVIDER: EMPIAR-11262 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Redβ<sub>177</sub> annealase structure reveals details of oligomerization and λ Red-mediated homologous DNA recombination.
Newing Timothy P TP Brewster Jodi L JL Fitschen Lucy J LJ Bouwer James C JC Johnston Nikolas P NP Yu Haibo H Tolun Gökhan G
Nature communications 20220926 1
The Redβ protein of the bacteriophage λ red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present the structure of a helical oligomeric annealing intermediate of Redβ, consisting of N-terminal residues 1-177 bound to two complementary 27mer oligonucleotides, determined via cryogenic electron microscopy (cryo-EM) to a final resolution of 3.3 Å. The structure reveals a continuous binding groove which positions and stabilizes ...[more]
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