Dataset Information
Cryo-electron tomography on plasma FIB lamellae of HeLa cells
Ontology highlight
ABSTRACT:
SUBMITTER: Michael Grange
PROVIDER: EMPIAR-11306 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Plasma FIB milling for the determination of structures in situ.
Nature communications 20230206 1
Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on gallium have been used. However, ion implantation, changes to surface chemistry and an inability to access high currents limit gallium application. Here, we show that plasma-coupled ion sources can produce cryogenic lamellae of vitrified human cells in a robust and automated manner, with quality sufficient for pseudo-atomic structure dete ...[more]