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Multishot Tomography for High-Resolution In Situ Subtomogram Averaging: E.coli cryo-FIB lamellae multishot

ABSTRACT:

SUBMITTER: Sagar Khavnekar

PROVIDER: EMPIAR-11325 | biostudies-other |

REPOSITORIES: biostudies-other

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Multishot tomography for high-resolution in situ subtomogram averaging.

Khavnekar S S Wan W W Majumder P P Wietrzynski W W Erdmann P S PS Plitzko J M JM

Journal of structural biology 20221104 1

Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields s ...[more]

PMID: 36343843

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