E.coli RNaseP Holoenzyme with Mg2+
Ontology highlight
ABSTRACT:
SUBMITTER: Wei Huang
PROVIDER: EMPIAR-11333 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.
Zhu Jiaqiang J Huang Wei W Zhao Jing J Huynh Loc L Taylor Derek J DJ Harris Michael E ME
Nature communications 20220831 1
Binding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES*). However, the structures of intermediates and the conformational changes that occur during binding are poorly understood. Here, we show that pairing between the 5' leader and 3'RCCA extending the acceptor stem of ptRNA inhibits ES* formation. Cryo-electron microscopy single particle analysis reveals a dynamic enzyme that becomes ordered up ...[more]
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