Dataset Information
Mouse heavy chain apoferritin in vitreous ice after plunge-freezing
Ontology highlight
ABSTRACT:
SUBMITTER: Ulrich Lorenz
PROVIDER: EMPIAR-11345 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Near-atomic resolution reconstructions from in situ revitrified cryo samples.
Acta crystallographica. Section D, Structural biology 20230523 Pt 6
A microsecond time-resolved version of cryo-electron microscopy (cryo-EM) has recently been introduced to enable observation of the fast conformational motions of proteins. The technique involves locally melting a cryo sample with a laser beam to allow the proteins to undergo dynamics in the liquid phase. When the laser is switched off, the sample cools within just a few microseconds and revitrifies, trapping particles in their transient configurations, in which they can subsequently be imaged. ...[more]