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Single particle cryo-EM dataset for E. coli MlaFEDB bound to MlaC

ABSTRACT:

SUBMITTER: Gira Bhabha

PROVIDER: EMPIAR-11354 | biostudies-other |

REPOSITORIES: biostudies-other

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Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning.

MacRae Mark R MR Puvanendran Dhenesh D Haase Max A B MAB Coudray Nicolas N Kolich Ljuvica L Lam Cherry C Baek Minkyung M Bhabha Gira G Ekiert Damian C DC

The Journal of biological chemistry 20230425 6

The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer that protects the cell from external stressors, such as antibiotics. The Mla transport system is implicated in the Maintenance of OM Lipid Asymmetry by mediating retrograde phospholipid transport across the cell envelope. Mla uses a shuttle-like mechanism to move lipids between the MlaFEDB inner membrane complex and the MlaA-OmpF/C OM complex, via a periplasmic lipid-binding protein, MlaC. MlaC binds to MlaD and MlaA, but ...[more]

PMID: 37100290

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