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The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase Wzz form a complex in vivo and in vitro


ABSTRACT:

SUBMITTER: David Bhella 

PROVIDER: EMPIAR-11355 | biostudies-other |

REPOSITORIES: biostudies-other

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The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex.

Weckener Miriam M   Woodward Laura S LS   Clarke Bradley R BR   Liu Huanting H   Ward Philip N PN   Le Bas Audrey A   Bhella David D   Whitfield Chris C   Naismith James H JH  

Open biology 20230322 3


The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the <i>Enterobacteriaceae</i>. ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase superfamily. WzyE activity is regulated by a membrane protein polysaccharide co-polymerase, WzzE. Förster resonance energy transfer experiments demonstrate that WzyE and WzzE from <i>Pectobacterium atrosepticum</i> form a complex <i>in vivo  ...[more]

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