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Movies for human polymerase alpha-primase in DNA elongation states

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SUBMITTER: Ci Ji Lim 

PROVIDER: EMPIAR-11361 | biostudies-other |

REPOSITORIES: biostudies-other

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Structures of human primosome elongation complexes.

He Qixiang Q   Baranovskiy Andrey G AG   Morstadt Lucia M LM   Lisova Alisa E AE   Babayeva Nigar D ND   Lusk Benjamin L BL   Lim Ci Ji CJ   Tahirov Tahir H TH  

Nature structural & molecular biology 20230417 5

The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-  ...[more]

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