Dataset Information
Movies for human polymerase alpha-primase in DNA elongation states
Ontology highlight
ABSTRACT:
SUBMITTER: Ci Ji Lim
PROVIDER: EMPIAR-11361 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structures of human primosome elongation complexes.
Nature structural & molecular biology 20230417 5
The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA- ...[more]