Dataset Information
Cryo serial FIB/SEM of RPE-1 cells
Ontology highlight
ABSTRACT:
SUBMITTER: Maud Dumoux
PROVIDER: EMPIAR-11421 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-plasma FIB/SEM volume imaging of biological specimens.
eLife 20230221
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in cells and tissues while maintaining constituents' hydration shells for in situ structural biology downstream. However, the application of serial FIB/SEM imaging of non-stained cryogenic biological samples is limited due to low contrast, curtaini ...[more]