KpFtsZ–Mb double helical tube
Ontology highlight
ABSTRACT:
SUBMITTER: Junso Fujita
PROVIDER: EMPIAR-11425 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structures of a FtsZ single protofilament and a double-helical tube in complex with a monobody.
Fujita Junso J Amesaka Hiroshi H Yoshizawa Takuya T Hibino Kota K Kamimura Natsuki N Kuroda Natsuko N Konishi Takamoto T Kato Yuki Y Hara Mizuho M Inoue Tsuyoshi T Namba Keiichi K Tanaka Shun-Ichi SI Matsumura Hiroyoshi H
Nature communications 20230710 1
FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic insights are lacking. Here, we determine the cryoEM structure of a single protofilament of FtsZ from Klebsiella pneumoniae (KpFtsZ) in a polymerization-preferred conformation. We also develop a monobody (Mb) that binds to KpFtsZ and FtsZ from Escherichia coli without affecting their GTPase activity. C ...[more]
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