Dataset Information
Cryo-EM particle stacks of the ∆KsgA immature 30S sample
Ontology highlight
ABSTRACT:
SUBMITTER: Joseph Davis
PROVIDER: EMPIAR-11529 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
KsgA facilitates ribosomal small subunit maturation by proofreading a key structural lesion.
Nature structural & molecular biology 20230831 10
Ribosome assembly is orchestrated by many assembly factors, including ribosomal RNA methyltransferases, whose precise role is poorly understood. Here, we leverage the power of cryo-EM and machine learning to discover that the E. coli methyltransferase KsgA performs a 'proofreading' function in the assembly of the small ribosomal subunit by recognizing and partially disassembling particles that have matured but are not competent for translation. We propose that this activity allows inactive parti ...[more]